BioThema*專(zhuān)注于螢火蟲(chóng)熒光素酶反應(yīng)的分析應(yīng)用，無(wú)論是檢測(cè)細(xì)胞，測(cè)量酶活性還是其他東西。在該反應(yīng)中，用ATP（三磷酸腺苷）活化熒光素，然后氧化成氧化熒光素和CO 2。氧化熒光素以激發(fā)態(tài)形成，并在進(jìn)入其基態(tài)時(shí)發(fā)光。通常在所謂的發(fā)光計(jì)中測(cè)量光。
ATP + 熒光素+ O 2 -熒光素酶 - > AMP +焦磷酸+氧化熒光素+ CO 2 +光
在ATP的測(cè)定中，光發(fā)射可以基本上恒定或快速衰減。因此，基于螢火蟲(chóng)熒光素酶反應(yīng)，存在穩(wěn)定的光和閃光型ATP試劑。在熒光素酶水平低的情況下，每分鐘消耗的ATP比例可以忽略不計(jì)，然后光可以幾乎恒定但也很低。在高水平的熒光素酶下，ATP被消耗是不可避免的，導(dǎo)致光迅速減少，但初峰值光很高。實(shí)際上，峰高和衰減率都與熒光素酶活性成比例。BioThema開(kāi)發(fā)了三種ATP試劑
BioThema2019年價(jià)格表
貨號(hào) | 品名 | 規(guī)格 | 價(jià)格 |
111-021 | Kinase RR Kit | 1000tests | 4879 |
144-041 | ATP Kit SL | 1000tests | 6035 |
155-050 | Cellular ATP Kit HTS | 1200tests | 7463 |
188-441 | Cell Viability Kit SL | 1600tests | 13073 |
266-111 | Intracellular ATP Kit HS | 375tests | 7769 |
266-112 | Microbial ATP Kit HS | 375tests | 7939 |
266-221 | ATP Hygiene Kit HS (dropper bottles) | 100tests | 3842 |
266-311 | ATP Biomass Kit HS | 375tests | 6341 |
484-001 | Luciferase Assay Kit | 800tests | 6902 |
484-102 | Luciferase Assay Kit HTS | 4800tests | 34578 |
484-103 | Luciferase Assay Kit HTS | 4600tests | 34578 |
PHL-192 | Portable Hygiene Laboratory | 192tests | 6970 |
11-501 | ATP Reagent SL | 250tests | 1156 |
11-501-M | ATP Reagent SL (excl. Mg2+) | 250tests | 1156 |
12-101 | ATP Reagent HS | 100tests | 646 |
12-071 | ATP Reagent HS | 17tests | 493 |
13-101 | ATP Reagent SS | 100tests | 3451 |
51-101 | ATP Substrate | 100tests | 442 |
51-201 | ATP Substrate | 200tests | 799 |
61-101 | Luciferin Substrate | 100tests | 442 |
61-201 | Luciferin Substrate | 200tests | 799 |
71-101 | ATP Eliminating Reagent | 500tests | 799 |
BT11-100 | D-Luciferin | 100mg | 867 |
BT11-500 | D-Luciferin | 500mg | 2754 |
BT11-1000 | D-Luciferin | 1000mg | 4420 |
BT12-100 | L-Luciferin | 100mg | 3026 |
BT11-100 K | D-Luciferin K salt | 100mg | 867 |
BT11-1000 K | D-Luciferin K salt | 1000mg | 4420 |
BT11-100 Na | D-Luciferin Na salt | 100mg | 867 |
BT11-1000 Na | D-Luciferin Na salt | 1000mg | 4420 |
LA-10 | Firefly Luciferase (recomb., enhanced activity) | 10mg | 13787 |
47-051 | ATP Standard | 5mL | 340 |
46-051 | ATP Standard | 5mL | 340 |
45-051 | ATP Standard | 5mL | 340 |
48-072 | ATP Standard (dropper bottle) | 6.7mL | 527 |
21-101 | Tris-EDTA Buffer | 10ml | 153 |
21-103 | Tris-EDTA Buffer | 100ml | 782 |
21-500 | Tris-EDTA Buffer | 500ml | 2550 |
22-101 | Diluent B | 10ml | 340 |
22-072 | Diluent B (dropper bottle) | 6.7ml | 289 |
23-101 | Diluent C (SL) | 10ml | 340 |
24-101 | Diluent D | 10ml | 340 |
27-101 | ATP Free H2O | 10ml | 323 |
40-500 | ATP Diluent | 10ml | 425 |
31-072 | Extractant B/S (dropper bottle) | 6.7ml | 408 |
31-101 | Extractant B/S | 10ml | 595 |
31-103 | Extractant B/S | 100ml | 3043 |
31-500 | Extractant B/S | 500ml | 9163 |
20-121 | Lysing Diluent | 12ml | 408 |
28-101 | Cell Lysing Reagent | 10ml | 884 |
681000 | Cuvettes with 'stirring wings' | 1000pcs | 2380 |
61303 | LuciPac W | 100 tests | 4573 |
60331 | LuciPac Pen | 100 tests | 4573 |
| CheckLite 250 Plus | 250 tests | 6511 |
| CheckLite HS Set | 100 tests | 6511 |
| CheckLite CT150 | 150 tests | 7531 |
LA-11 | Firefly Luciferase (recomb., thermostable) | 10 mg | 6086 |
LA-12 | Firefly Luciferase (recomb., thermostable) | 100 mg | 24752 |
PD-20 | Lumitester™ handluminometer PD-20 | ea | 17697 |
PD-30 | Lumitester™ handluminometer PD-30 | ea | 18751 |
C-110 | Lumitester™ portable luminometer C-110 inc | ea | 86615 |
DPU-H245 | Printer set for LUMITESTER C-110 Includes following items | ea | 5661 |
BioThema中國(guó)代理,BioThema上海代理，BioThema北京代理，BioThema廣東代理，BioThema江蘇代理BioThema湖北代理,BioThema天津代理,BioThema黑龍江代理,BioThema湖南代理BioThema內(nèi)蒙古代理,BioThema吉林代理,BioThema福建代理,BioThema江蘇代理,BioThema浙江代理,BioThema四川代理,BioThema代理，
上海起發(fā)實(shí)驗(yàn)試劑有限公司是實(shí)驗(yàn)試劑一站式采購(gòu)服務(wù)商
1：強(qiáng)大的進(jìn)口輻射能力，血清、抗體、耗材、大部分限制進(jìn)口品等。
2：產(chǎn)品種類(lèi)齊全，經(jīng)營(yíng)超過(guò)700多品牌，基本涵蓋所有生物實(shí)驗(yàn)試劑耗材。
3：提供加急服務(wù)，貨品一般1-2周到貨。
4：富有競(jìng)爭(zhēng)良好的信譽(yù)，大部分客戶(hù)提供貨到付款服務(wù)?？蛻?hù)包括清華、北大、交大、復(fù)旦、中山等100多所高校，ROCHE，阿斯利康、國(guó)藥、fisher等知藥企。
6：我們還是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,BioThema,RESEARCH DIETS,INC,sterlitech；sysy,TriLink BioTechnologies,Inc；worthington-biochem,zyagen等幾十家國(guó)外公司授權(quán)代理。
7：我們還是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知*批發(fā)，歡迎合作。
