RNAscope® 原位雜交
RNAscope®技術(shù)是近年來zui火的RNA原位雜交技術(shù)，是RNA原位雜交（ISH）領(lǐng)域的一項(xiàng)重大進(jìn)步。由Advanced Cell Diagnostics公司（Newark, CA）開發(fā)，通過的雙“Z”探針設(shè)計(jì)和信號(hào)放大系統(tǒng)，使RNA原位雜交具有高度特異性、單分子檢測(cè)的敏感性并有*的信噪比，能夠在單細(xì)胞水平同時(shí)定量多個(gè)RNA的表達(dá)，在獲得單細(xì)胞中單拷貝RNA表達(dá)數(shù)據(jù)的同時(shí)提供完整的組織形態(tài)學(xué)信息。
RNAscope 方法克服了以前的RNA原位雜交技術(shù)的非特異性雜交、靈敏度低、對(duì)樣本要求高等弱點(diǎn)，可替代傳統(tǒng)的ISH/FISH RNA 原位檢測(cè)，同時(shí)單分子檢測(cè)靈敏度提供了在組織細(xì)胞原位對(duì)單個(gè)細(xì)胞中基因的表達(dá)進(jìn)行檢測(cè)，釋放RNA作為生物標(biāo)記的全部潛能，提高對(duì)疾病與標(biāo)志物之間復(fù)雜的生物學(xué)相關(guān)性的認(rèn)識(shí)，是理想的能夠用于NGS和芯片技術(shù)后期轉(zhuǎn)化研究技術(shù)平臺(tái)。
RNAscope® 原位雜交步驟：
RNAscope®原位雜交是一項(xiàng)新穎的用于檢測(cè)位于組織細(xì)胞原位的目標(biāo)RNA的原位雜交（In Situ Hybridization，ISH)檢測(cè)技術(shù)。該技術(shù)進(jìn)行RNA原位雜交檢測(cè)主要分成5步。
Step 1：透化：通過RNAscope®預(yù)處理試劑盒處理載玻片上固定好的組織或細(xì)胞，暴露目標(biāo)RNA。
Step 2：探針雜交：針對(duì)靶基因設(shè)計(jì)RNAscope®Z型探針與目標(biāo)RNA雜交。
Step 3：信號(hào)放大：RNAscope®檢測(cè)試劑盒逐級(jí)信號(hào)放大。
Step 4：信號(hào)可視化：在普通光學(xué)顯微鏡或者多光譜熒光成像系統(tǒng)下，每一個(gè)目標(biāo)RNA分子以一個(gè)點(diǎn)狀信號(hào)的形式呈現(xiàn)。
Step 5：量化分析：顯微鏡下直接計(jì)數(shù)或者使用圖像分析軟件如RNAscope® SpotStudio™ 或HALO對(duì)每一個(gè)細(xì)胞中的RNA單分子信號(hào)進(jìn)行定量分析。
acdbio 為您提供RNAscope® 原位雜交所需的RNAscope® Probes, Reagent Kits, Hybridization Systems, Software & Quality Controls: end-to-end solutions for your RNA ISH work.
acdbio具體產(chǎn)品信息如下：
| 貨號(hào) | 產(chǎn)品名稱 | 品牌 |
| 300063 | RNAscope SpotStudio v1.0 Software (Trial Pack - 15 ROIs) | ACDBIO |
| 300060 | RNAscope SpotStudio v1.0 Software ( 3000 ROIs) | ACDBIO |
| 300062 | RNAscope SpotStudio v1.0 Software ( 150 ROIs) | ACDBIO |
| 310019 | ACD EZ-Batch Wash Tray | ACDBIO |
| 310017 | ACD EZ-Batch Slide Holder | ACDBIO |
| 310007 | ACD EZ-Batch Slide Processing System | ACDBIO |
| 310015 | ACD HybEZ Humidifying Paper (15 sheets) | ACDBIO |
| 310014 | ACD HybEZ Slide Rack | ACDBIO |
| 310012 | ACD HybEZ Humidity Control Tray | ACDBIO |
| 310013 | ACD HybEZ Hybridization System (220v) | ACDBIO |
| 310010 | ACD HybEZ Hybridization System (110v) | ACDBIO |
| 321461 | ACD HybEZ Hybridization System (220v) | ACDBIO |
| 321462 | ACD HybEZ Hybridization System (220v) | ACDBIO |
| 320409 | EcoMount (BioCare) | ACDBIO |
| 310018 | ImmEdge Hydrophobic Barrier Pen | ACDBIO |
| 310023 | RNAscope Control Slide-Mouse 3T3 Cell Pellet | ACDBIO |
| 310045 | RNAscope Control Slide-Human Hela Cell Pellet | ACDBIO |
| 300041 | RNAscope Blank Probe - C1 | ACDBIO |
| 320630 | RNAscope VS Accessory Kit | ACDBIO |
| 322000 | RNAscope Target Retreival Reagents | ACDBIO |
| 322380 | RNAscope 2.5 Universal Pretreatment Reagents | ACDBIO |
| 310091 | RNAscope Wash Buffer Reagents | ACDBIO |
| 322330 | RNAscope H202 & Protease Plus Reagents | ACDBIO |
| 320970 | RNAscope PBMC Sample Prep Reagents | ACDBIO |
| 322340 | RNAscope Protease III & Protease IV Reagents | ACDBIO |
| 320851 | RNAscope Multiplex Fluorescent Detection Reagents | ACDBIO |
| 320701 | RNAscope 2-Plex Detection Reagents | ACDBIO |
| 322360 | RNAscope 2.5 HD Detection Reagents-RED | ACDBIO |
| 322310 | RNAscope 2.5 HD Detection Reagents-BROWN | ACDBIO |
| 322371 | RNAscope Intro. Pack for 2.5 HD Reagent Kit Brown | ACDBIO |
| 322370 | RNAscope Intro. Pack for 2.5 HD Reagent Kit Brown | ACDBIO |
| 322150 | RNAscope 2.5 LS Reagent Kit-RED | ACDBIO |
| 322100 | RNAscope 2.5 LS Reagent Kit-BROWN | ACDBIO |
| 322250 | RNAscope 2.5 VS Reagent Kit-RED | ACDBIO |
| 322200 | RNAscope 2.5 VS Reagent Kit-BROWN | ACDBIO |
| Custom | RNAscope Multiplex Fluorescent Reagent Kit (4-plex) | ACDBIO |
| 320850 | RNAscope Multiplex Fluorescent Reagent Kit | ACDBIO |
| 320700 | RNAscope 2-plex Reagent Kit | ACDBIO |
| 322360 | RNAscope 2.5 HD Reagent Kit- RED | ACDBIO |
| 322300 | RNAscope 2.5 HD Reagent Kit- BROWN | ACDBIO |
| 310043-C3 | RNAscope Negative Control Probe - DapB-C3 | ACDBIO |
| 310043-C2 | RNAscope Negative Control Probe - DapB-C2 | ACDBIO |
| 320891 | RNAscope 3-plex Positive Control Probe - Rn | ACDBIO |
| 320881 | RNAscope 3-plex Positive Control Probe - Mm | ACDBIO |
| 320861 | RNAscope 3-plex Positive Control Probe - Hs | ACDBIO |
| 320771 | RNAscope 2-plex Positive Control Probe - Rn | ACDBIO |
| 320761 | RNAscope 2-plex Positive Control Probe - Mm | ACDBIO |
| 320741 | RNAscope 2-plex Positive Control Probe - Hs | ACDBIO |
| 313918 | RNAscope 2.5 LS Positive Control Probe - Mm-PPIB | ACDBIO |
| 313908 | RNAscope 2.5 LS Positive Control Probe - Hs-PPIB | ACDBIO |
| 312038 | RNAscope 2.5 LS Negative Control Probe - DapB | ACDBIO |
| 300038 | RNAscope 2.5 LS Target Probes | ACDBIO |
| 313909 | RNAscope 2.5 VS Positive Control Probe - Hs-PPIB | ACDBIO |
| 312039 | RNAscope 2.5 VS Negative Control Probe - DapB | ACDBIO |
| 300039 | RNAscope 2.5 VS Target Probes | ACDBIO |
| 300031-C3 | RNAscope Probe- New Target Probe C3 | ACDBIO |
| 300031-C2 | RNAscope Probe- New Target Probe C2 | ACDBIO |
| 300031 | RNAscope Probe- New Target Probe C1 | ACDBIO |
| 320269-C3 | Setup Fee For New Target (Standard C3 probe) | ACDBIO |
| 320269-C2 | Setup Fee For New Target (Standard C2 probe) | ACDBIO |
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